Resources and Equipment | Proteomics
Resources and Equipment
The equipment is mainly distributed across rooms I-1/866, I-1/867, and F-1/800 at Son Espases University Hospital.
The platform also includes auxiliary equipment that complements experimental workflows, such as: Trans-Blot Turbo Transfer System (Bio-Rad), EPS-601 Electrophoresis Power Supplies (General Electric), Mini VE Vertical Electrophoresis System (General Electric), Ettan Dalt Six Two-Dimensional Electrophoresis System (General Electric), Ettan IPGphor 3 Isoelectric Focusing System (General Electric), Duomax 1030 Rocking Shaker (Heidolph).
These instruments are distributed across different laboratories within the Institute, facilitating the development of various stages of proteomic analysis.
LAS 4000
|
Room |
Technology Type | Detection Mode | Dynamic Range | Resolution | Light Source | Main Applications |
|---|---|---|---|---|---|---|
|
I-1/867 |
Digital imaging of chemiluminescence and fluorescence | Chemiluminescence, fluorescence, visible light | Very wide | High | Visible light / fluorescence / chemiluminescence | Western blot, protein expression, biomarkers, post-translational modifications |
FLA 9500
|
Room |
Technology Type | Detection Mode | Dynamic Range | Resolution | Light Source | Main Applications |
|---|---|---|---|---|---|---|
|
I-1/866 |
Multimodal laser scanner | Multichannel fluorescence, radioisotopes, chemiluminescence | Wide | Very high | Multichannel lasers (Cy3, Cy5, etc.) | 2D-DIGE, differential expression, protein validation, multiplexing |
Odyssey CLX
|
Room |
Technology Type | Detection Mode | Dynamic Range | Resolution | Light Source | Main Applications |
|---|---|---|---|---|---|---|
|
I-1/866 |
Dual infrared imaging | Near-infrared fluorescence (700/800 nm) | Very wide | High | Near-infrared | Multiplexed Western blot, precise quantification, phosphorylation studies |
Luminex MAGPIX
|
Room |
Technology Type | Detection Mode | Dynamic Range | Resolution | Light Source | Main Applications |
|---|---|---|---|---|---|---|
|
I-1/867 |
Multiplexing via fluorescent microspheres | Multiplexed fluorescence in microspheres | High | Medium | LED / internal fluorescence | Cytokines, growth factors, biomarkers, immunology |
Biotek Synergy H1
|
Room |
Technology Type | Detection Mode | Dynamic Range | Resolution | Light Source | Main Applications |
|---|---|---|---|---|---|---|
|
F-1/800 |
Multimodal microplate reader | Absorbance, fluorescence, luminescence | Variable by mode | High | LED / xenon lamp | ELISA, enzymatic assays, protein-protein interaction, cell viability |
NanoSight NS300
|
Room |
Technology Type | Detection Mode | Dynamic Range | Resolution | Light Source | Main Applications |
|---|---|---|---|---|---|---|
|
I-1/867 |
Nanoparticle Tracking Analysis (NTA) |
Light scattering for particle tracking | Real per particle | High (single particle) | Scattered light laser | Exosomes, extracellular vesicles, protein aggregates, quality control |
Odyssey® M
|
Room |
Technology Type | Detection Mode | Dynamic Range | Resolution | Light Source | Main Applications |
|---|---|---|---|---|---|---|
|
F-1/800 |
Advanced multimodal imaging | Fluorescence (IR and visible), chemiluminescence, RGB | Very wide | Up to 5 microns | Multichannel: IR, visible, RGB, chemiluminescence | Western blot, ELISA, cell cultures, gel and tissue documentation, multiplexing |
Equipments
