Additional Information | Flow Cytometry and Cell Biology

Information of Interest 

The main applications that IdISBa offers in the field of cytometry and cell biology are detailed below.

Flow Cytometry

  • Sample preparation for flow cytometry
  • Immunophenotyping (surface or intracellular)
  • Cell cycle and functionality studies
  • Proliferation
  • Apoptosis
  • Cell sorting

Cell Culture:

  • Use of incubators and biosafety cabinets for primary cultures and maintenance of cell lines
  • Tissue dissociation
  • Electroporation
  • Cell counting
  • RTCA
  • Cryopreservation of cells and cell lines in liquid nitrogen

Cellular Metabolism: 

Analysis of cellular metabolism: oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in real time, in a 96‑well plate format.

3D Bioprinting: 

  • Generation of organoids and spheroids.
  • Printing of 3D tissue and organ models.
  • Development of organs‑on‑a‑chip.
  • Deposition of cells and biomolecules with micrometric precision (micropatterning).
  • Printing with hydrogels and multimaterial formats.

General Services:

  • Training users in the handling of available equipment.
  • Technical advice on experimental design, sample preparation, etc.